A Fluorescence-based Assay of Phospholipid Scramblase Activity
نویسندگان
چکیده
منابع مشابه
Constitutive phospholipid scramblase activity of a G Protein-coupled receptor*
Opsin, the rhodopsin apoprotein, was recently shown to be an ATP-independent flippase (or scramblase) that equilibrates phospholipids across photoreceptor disc membranes in mammalian retina, a process required for disc homoeostasis. Here we show that scrambling is a constitutive activity of rhodopsin, distinct from its light-sensing function. Upon reconstitution into vesicles, discrete conforma...
متن کاملPhospholipid scramblase 1 potentiates the antiviral activity of interferon.
Phospholipid scramblase 1 (PLSCR1) is an interferon (IFN)- and growth factor-inducible, calcium-binding protein that either inserts into the plasma membrane or binds DNA in the nucleus depending on its state of palmyitoylation. In certain hematopoietic cells, PLSCR1 is required for normal maturation and terminal differentiation from progenitor cells as regulated by select growth factors, where ...
متن کاملCalcium-dependent Phospholipid Scramblase Activity of TMEM16 Family Members*
Background: TMEM16A and 16B work as Cl-channel, while 16F works as phospholipid-scramblase. Function of other TMEM16 members is unknown. Results: Using TMEM16F cells, TMEM16C, 16D, 16F, 16G and 16J were shown to be lipid scramblases. Conclusion: Some TMEM16 members are divided into two Cl-channels and five lipid-scramblases. Significance: Learning biochemical function of TMEM16 family members i...
متن کاملCalcium-dependent phospholipid scramblase activity of TMEM16 protein family members.
BACKGROUND TMEM16A and 16B work as Cl(-) channel, whereas 16F works as phospholipid scramblase. The function of other TMEM16 members is unknown. RESULTS Using TMEM16F(-/-) cells, TMEM16C, 16D, 16F, 16G, and 16J were shown to be lipid scramblases. CONCLUSION Some TMEM16 members are divided into two Cl(-) channels and five lipid scramblases. SIGNIFICANCE Learning the biochemical function of...
متن کاملDirect fluorescence-based lipase activity assay.
Lipases and esterases (glycerol ester hydrolases, E.C. 3.1.1.-) are enzymes acting on the carboxyl ester bonds present in acylglycerols. They have been recognized as useful biocatalysts because of their wide-ranging versatility in industrial applications (2,5). However, the physical properties of lipids have caused a great difficulty in studying lipolytic enzymes (4). Traditionally, lipases hav...
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ژورنال
عنوان ژورنال: Journal of Visualized Experiments
سال: 2016
ISSN: 1940-087X
DOI: 10.3791/54635